USMLE Step 1 Lecture Notes 2016: Biochemistry and Medical by Kaplan

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5’ 5’ 5’ 2 1 3’ 3’ 1 3’ 2 5’ 1 3’ 3’ 1 Lagging Strand Synthesis (Discontinuous) 1. Primase synthesizes the primer ( ) 5’ to 3’. 4. As helicase opens more of the replication fork, a third Okasaki fragment will be added. 5’ 5’ 2 ) continues to unwind the DNA. 3. Synthesis stops when DNA polymerase encounters the primer of the leading strand on the other side of the diagram (not shown), or the primer of the previous (Okasaki) fragment. 3’ 3’ 3’ 3. Helicase ( 2. DNA polymerases α and δ extend the primer, moving away from the replication fork (Lagging strand synthesis).

Com Chapter 3 Transcription and RNA Processing ● DNA Coding strand 5' A T G G G G C T C A G C G A C 3' Transcription 3' T A C C C C G A G T C G C T G 5' Template strand mRNA Translation DNA coding strand is identical to the mRNA (except T for U) DNA template strand is complementary and antiparallel to the mRNA 5' A U G G G G C U C A G C G A C 3' Protein NH2 Codons Met Gly Leu Ser Asp COOH Direction of transcription Direction of translation Figure I-3-3.  During RNA synthesis, the DNA template sequence TAGC would be transcribed to produce which of the following sequences?

Com Chapter 2 DNA Replication and Repair ● Similarities include: ● The newly synthesized strand is made in the 5′→3′ direction. ● The template strand is scanned in the 3′→5′ direction. ● The newly synthesized strand is complementary and antiparallel to the template strand. ● Each new nucleotide is added when the 3′ hydroxyl group of the growing strand reacts with a nucleoside triphosphate, which is base-paired with the template strand. Pyrophosphate (PPi, the last two phosphates) is released during this reaction.

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