By James H. Cyriax MD MRCP, P. J. Cyriax MCSP
New in paperback layout, this version covers the method and strategies of 1 of the founding figures within the box of Orthopaedic drugs. Presentation and content material were thoroughly up to date and revised for the fashionable practitioner.
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WAGEMAKER can be achieved by means of electroporation , calcium phosphate precipitation, lipofection, or use of plasmids and Adv constructs [46, 161]. Adenoviral-mediated infections have the advantage over the use of retroviruses since they do not require cell divisions for gene insertion and have a low toxicity. As a corollary, multiple copies of the intended gene can be inserted into the genome of the target cell. However, the number of copies transferred to the transfected MSCs cannot be controlled and is therefore highly variable and unpredictable.
Recently a study was published by Campagnoli and colleagues, who reported that MSCs could be derived from fetal tissues, such as blood, liver and bone marrow, from as early as eight weeks of gestation. They demonstrated that these cells could be relatively easy retrovirally transduced, with more than 99% of the cells expressing enhanced GFP (EGFP) at high levels, without any kind of selection . They suggested that MSCs could therefore also serve as suitable vehicles for the prenatal delivery of certain genes and even more boldly, that genetically modiﬁed autologous fetal MSCs could be possibly used as an alternative treatment modality for genetic disorders, known to cause irreversible damage before birth, when transplanted in utero.
Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol. 1999;181:67–73. 18 Fuchs E, Segre J. Stem cells: a new lease on life. Cell. 2000;100:143–155. 19 Watt FM, Hogan BLM. Out of Eden: stem cells and their niches. Science. 2000;287:1427–1430. 20 Tavassoli M, Crosby WH. Transplantation of marrow to extramedullary sites. Science. 1968;161(836):54– 6. 21 Fernandez M, Simon V, Herrera G, Cao C, Del Favero H, Minguell JJ. Detection of stromal cells in peripheral blood progenitor cell collections from breast cancer patients.